Nanophotonics based label free detection mechanism for real-time monitoring of interleukin-6.

Magneto-photonic crystals/MPCs are promising candidates for devising high-fidelity embedded biosensor systems which offer facile & real time detection of diagnostic proteins. Despite extensive use of magnetic nanomaterials for theranostic applications, the idea of exploiting its photonic response when assembled as a colloid inside a matrix remains unexplored. Herein, we report a novel label free method for quantitative detection of interleukin 6 which is a widely used prognostic marker for multiple pathological conditions. Cobalt ferrite/CoF and magnetite nanoparticles with Ms of 74.8 and 77 emu g-1 were assembled inside a hydrogel matrix with the application of an external magnetic field. Through the use of click chemistry, detecting antibodies were immobilized on their surface. The interaction of interleukin 6 with the antibodies produces a blue-shift in resonant wavelength and the reflectance intensity increases up to 50% and 44% when tested with CoF & magnetite based MPC respectively at a concentration of 50 pg ml-1. The dynamic range of the sensor lies within the prognostic values of IL-6, and the integrated sensing mechanism proposed in this study provides an ideal platform for real-time management of sepsis in patients with higher degree burns.

A review on recent advances in chitosan based composite for hemostatic dressings.

High mortality rate in potentially survivable casualties due to severe hemorrhage is a major challenge in today's battlefield because technological advancements have revolutionized the combat tactics and complicated the type and severity associated with wound grades. Quality of pre-hospital care prior to patient evacuation is crucial in determining the survival rate in injured patients. To deal with this challenge, considerable improvements in the hemostatic dressings have been introduced and pre-hospital care has been upgraded in many tactical combat casually care guidelines. Combat Gauze has been widely used bandage which is now been replaced by different chitosan based hemostatic dressings. It not only exhibits anti-bacterial activity but also induces hemostasis via direct interaction with erythrocytes and platelets. Its hemostasis mechanism is not dependent on host coagulation pathway which makes it an ideal dressing to stop bleeding in coagulopathic patients. Different generations of chitosan bandages have been developed to overcome the limitations of previous ones. This review provides performance analysis of chitosan bandage generations and discusses the progress made in its fabrication methods during the recent years.